LOCUS       40924_23894       12051 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB768 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12051)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Gateway Binary Vectors with Organelle-Targeted Fluorescent Proteins 
            for Highly Sensitive Reporter Assay in Gene Expression Analysis of 
            Plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12051)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane 
            University, Interdisciplinary Center for Science Research; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12051)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12051)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane University, 
            Interdisciplinary Center for Science Research; 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             2065..2781
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      2815..3067
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(3149..3179)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3194..3215)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3251..3503)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(4888..5067)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5573..5597)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6118..6906
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7155..7743
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7929..8069)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8413..8607)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8676..9746)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10178..10804)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     protein_bind    complement(join(11930..12051,1))
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatggtg agcaagggcg aggagctgtt caccggggtg
     2101 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     2161 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     2221 aagctgcccg tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc
     2281 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2341 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2401 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2461 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2521 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     2581 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2641 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     2701 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactcac
     2761 ggcatggacg agctgtacaa gtcgaagcta tgagcttaga gctcgaattt ccccgatcgt
     2821 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2881 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2941 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     3001 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     3061 ctagatcggg aattggttcc ggaaccaatt cgtaatcatg gtcatagctg tttcctgtgt
     3121 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3181 cctggggtgc ctaatgagtg agctaactca cattaggctg aattaggcgc gcctatttct
     3241 attaattccc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
     3301 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
     3361 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
     3421 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
     3481 tattgccaaa tgtttgaacg atcggggaaa ttcgcctcga gtgacttctc tgattgcaga
     3541 ctatttatac caaccagcaa gaaagtgtcg aagtatgaag cagaaggcgc aggctagagc
     3601 ctggaaaacg agaagatgga tgcaaagaga cttttctgat ttaggaccaa acaacctcaa
     3661 gtaaacattg acgagtgtcc catcttttgt gccggtcagt aatccggtag caggatcata
     3721 ccttgggaga cgatggcgcg gacatttcac aatcccagca agctgaggaa gcgataatag
     3781 aaggttcaga acttgaggaa gaaagaagat gaggagggtt tcactgaagt gacccagaat
     3841 accaacaact gccatagtca ttccggcgaa gactgtgtaa gtgtctccaa caaaaactga
     3901 agaaggatac caattgtaag caagcattgc caaggatgtg gccatcagcg gttgagtaag
     3961 aaatatcgag aaggcatgag cttggtgata ctcgggatca acagatgctc cgatttgcat
     4021 aacgttgtgt atcaggatag cagcggcaat aacaactgtt tgaccgattt caaggccatt
     4081 cagaccagcg tggatgttaa tagagtttgt acaaaagaca gcaagtagcc ccatatataa
     4141 cttatatatc cttcctagat taagcacttc caagccaata taagcaacaa gaggttttgg
     4201 tataacaata gttgtatgac cagcataagc catcaaaagt ggaagggtag caaaagatgg
     4261 caggacaagt tttactctcc aaggcacatc aaggacatca tctacaaatc caagcaaaat
     4321 catgaagcaa atagatgcta gtgctgcatt atactccaca agccaattag aatcttcagt
     4381 gaagttaaaa tactgaaata ttatcgccac gatcaaaaag acaataccaa caacaatacc
     4441 caatgactct ggcactttaa tatctccttg aggcgtgcct cttttgttga tatcaaaacc
     4501 aaacatgtta cgtctcagaa cgtatctggc agtaacagga atcattttga gagtgacgaa
     4561 aaacccaacc aagctaagcc ccgcgttaat tagaatcgat cgcttgagtt cattatcaac
     4621 cttgtagtga aaacagagaa ggtatagata gagcgaacag agaagagtag agatcacgaa
     4681 gatcactcct aatttcggcg gcgcgagacg aaattcctca ccggaagcag aaaccgtctt
     4741 tcgggtcatc ttctgctccg atggcgctga gttgggttcc gacgggtccg gtttgttggg
     4801 aattgagatc gatgaagctc tcttacgagc tgccatatct tattgccccc cgggatcaga
     4861 tctggattga gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc
     4921 agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga
     4981 acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc
     5041 tgagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc
     5101 gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc ttgatcccct
     5161 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
     5221 cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca
     5281 gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt
     5341 ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg
     5401 actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca
     5461 gcgtgaagct gatccgtcga gattaataga aataaattca gcctaattcg gcgttaattc
     5521 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     5581 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     5641 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     5701 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     5761 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     5821 tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     5881 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     5941 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     6001 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     6061 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     6121 ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     6181 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     6241 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     6301 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     6361 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     6421 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     6481 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     6541 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     6601 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     6661 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     6721 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     6781 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     6841 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     6901 ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     6961 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
     7021 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc
     7081 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7141 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7201 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7261 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     7321 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7381 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7441 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7501 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7561 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7621 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7681 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7741 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7801 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7861 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7921 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     7981 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     8041 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     8101 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8161 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
     8221 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
     8281 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
     8341 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
     8401 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
     8461 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
     8521 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
     8581 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
     8641 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
     8701 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
     8761 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
     8821 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
     8881 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
     8941 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
     9001 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
     9061 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
     9121 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
     9181 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
     9241 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
     9301 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
     9361 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
     9421 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
     9481 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
     9541 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
     9601 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
     9661 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
     9721 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
     9781 tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
     9841 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
     9901 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
     9961 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
    10021 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
    10081 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
    10141 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
    10201 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
    10261 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
    10321 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
    10381 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
    10441 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
    10501 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
    10561 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
    10621 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
    10681 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
    10741 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
    10801 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
    10861 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
    10921 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
    10981 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
    11041 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
    11101 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
    11161 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
    11221 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
    11281 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
    11341 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
    11401 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
    11461 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
    11521 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
    11581 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
    11641 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
    11701 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
    11761 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
    11821 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
    11881 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
    11941 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga
    12001 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc t
//
